The Study of Molecular Detection Systems of PCR-Based Biological Threats with Field Applications

Document Type : Original Article

Authors

1 tehran university

2 imam hossein university

Abstract

Accurate and rapid diagnosis of biological threats has an important role in reducing vulnerability, sustaining essential activities, promoting national sustainability and facilitating crisis management in confronting these threats. The diagnostic systems of biological threats will lead to effectiveness in the passive biological defense. Currently, in the field of rapid and accurate detection of biological agents, various systems have been designed and constructed which are descriptive-analytic and based on functional characteristics, the most important ones of which have been introduced. By studying the approach of different countries, molecular diagnostic methods of biological agents due to high accuracy and speed have been considered and focused upon. The results of this research show that among various diagnostic systems of biological agents, high accuracy and high speed molecular diagnostic methods with field/operational applications, have high priority in the development and acquisition of biological threat detection technologies that can play an effective role in passive defense.

Keywords

References

  1. 1. مینایی، محمد ابراهیم، سعادتی، مجتبی، نجفی، مصطفی، هنری، حسین، نظریان، شهرام، نانوبیوسنسورها ابزاری برای تشخیص عوامل بیولوژیک در تهدیدات بیولوژیکی و بیوتروریستی، فصلنامه پدافند غیرعامل، شماره 12، ص 11، زمستان 1391.##

    2. مینایی، محمد ابراهیم، حسین زاده، مهدی، باقری پور، محمدجواد، تأثیر پدافند غیرعامل در حوزه پدافند جنگ نوین NBC، فصلنامه پدافند غیرعامل، شماره 7، ص 37، 1390.##

    1. A. A. Fatah, “An Introduction to Biological Agent Detection Equipment for Emergency First Responders,” National Institute of Justice, NIJ Guide 101−00, 2015.##
    2. B. D. Mariani, D. S. Martin, A. F. Chen, H. Yagi, S. S. Lin, and R. S. Taun, “PCR-Molecular diagnostic Technology for Monitoring Chronic Osteomyelitis,” Journal of Experimental Orthopaedics, vol. 19, 2014.##
    3. R. M. Ozanich, R. A. Bartholomew, J. S. Arce, C. J. Bruckner-Lea, and H. C. Cardamone, “Biodetection Technologies for First Responders,” 2015.##
    4. Y. Wang, Z. Wang, C. Liu, A.H. Yu, “PCR-based assays for the diagnosis of human Brucellosis, Annuals of Clinical Microbiology & Antomicrobials,” 13:31, 2014.##
    5. D. R. Seiner, H. A. Colburn, C. Baird, R. A. Bartholomew, T. Straub, K. Victry, et al., “Evaluation of the FilmArray(R) system for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis,” Journal of applied microbiology, vol. 114, no. 4, pp. 992-1000, 2013.##
    6. U. K. Spaulding, C. J. Christensen, R. J. Crisp, M. B. Vaughn, R. C. Trauscht, J. R. Gardner, et al., “RAZOR EX anthrax air detection system,” J. AOAC Int., vol. 95, no. 3, pp. 860-91, 2012##.
    7. T. Hadfield, V. Ryan, U. K. Spaulding, K. M. Clemens, I. M. Ota, and S. L. Brunelle, “RAZOR EX Anthrax Air Detection System for detection of Bacillus anthracis spores from aerosol collection samples: collaborative study,” J. AOAC Int., vol. 96, no. 2, pp. 392-398, 2013.##
    8. Available from: https://biomeme.com/.##
    9. R. P. Wilkes, P.-YA. Lee, Y.-L. Tsai, C.-F. Tsai, H.-H. Chang, H.-FG. Chang, et al., “An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need, Journal of Virological Methods, vol. 220, pp. 8-35, 2015.##
    10. Available from: http://www.genereach.com.##
    11. S. D. Neeraja Venkateswaran, “Evaluation of A Rapid Portable Multiplex Molecular Test for Simultaneous Detection of Botulinum Neurotoxin a (BONT/A) and Botulinum Toxin B (BONT/B),” 2015.##
    12. Available from: https://www.tetracore.com.##
    13. R. M. Ozanich, H. A. Colburn, K. D. Victry, R. A. Bartholomew, J. S. Arce, A. Heredia-Langner, et al., “Evaluation of PCR Systems for Field Screening of Bacillus anthracis,” Health security, vol. 15, no. 1, pp.70-80, 2017.##
    14. Available from: https://www.smithsdetection.com/.##
Passive Defense
Volume 10, Issue 2 - Serial Number 38
September 2019
Pages 97-105

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History

  • Receive Date: 09 September 2018
  • Accept Date: 05 May 2019
  • Publish Date: 23 August 2019

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